Liver Regeneration after Partial Hepatectomy in Male Albino Rat; the Possible Enhancement effect of Fiesitin

Partial hepatectomy (PHx) is typically performed for purposes such as organ donation, tumor removal, or treatment of traumatic injury. The regenerative capacity of the remaining liver tissue plays a critical role in determining morbidity and mortality rates. Fisetin, a natural antioxidant and senolytic flavonoid, is known for its ability to promote regeneration in bone and skin. This research aims, for the first time, to investigate the effects of fisetin on liver regeneration following extensive PHx. Thirty mice were divided into four groups: sham, fisetin control, 70% partial hepatectomy (PHx), and PHx with fisetin administered at ten milligrams per kilogram daily via oral gavage for four days prior to and two days following the PHx. The mice were euthanized 48 hours after the PHx procedure. Serum alanine aminotransferase (ALT) levels and hepatic oxidative stress markers, specifically glutathione (GSH), were evaluated. Fisetin enhanced PHx-induced liver regeneration with a significant increase in mitotic index, relative liver weight, a reduction in the elevated histopathological score of serum ALT and an increase of hepatic GSH, indicating antioxidant activity. Fisetin exerts a proliferative, anti infilammatory and anti-oxidative stress on liver cells, so it can enhance PHx-induced liver regeneration.